Now that technology is here, I do not think it would be as much of a challenge to increase number of frames compared to original research. Also can this resolve what is going on in the cell? Would be quite a boon to protein folding research if we can see clearly how the process happens.
Nope because that is limited by the diffraction limit for white light or evescent propogation for FL. You might be able to come up with something clever with FRET but I dont see comming up with something clever being limited by fps speeds.